Using PCR-RFLP technology to teach single nucleotide polymorphism for undergraduates
نویسندگان
چکیده
منابع مشابه
A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not t...
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Alpha-lactalbumin (α-LA, LALBA) was strongly correlated with the nutritional value and the functional properties of whey and whey products. However, there are not many studies of goat LALBA gene and its polymorphisms in literary sources. In this paper, on the basis of PCR-SSCP and DNA sequencing, one M63868:g.1897T>C mutation in exon 3 at LALBA locus identified a Single Nucleotide Polymorphism ...
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A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different geographical origins (Japan, Italy, and Nepal) and hosts (humans, calves, and a goat). These isolates were characterized by means of DNA sequencing, PCR-restriction fragment length polymorphism (PCR-RFLP),...
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Article ID: WMC002401 Article Type: Research Protocol Submitted on:01-Nov-2011, 12:31:07 PM GMT Published on: 02-Nov-2011, 05:42:47 PM GMT Article URL: http://www.webmedcentral.com/article_view/2401 Subject Categories:GENETICS
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Background Single nucleotide polymorphism (SNPs) are considered as one of the underlying causes of male infertility. Proper sperm chromatin packaging which involves replacement of histones with protamines has profound effect on male fertility. Over 20 SNPs have been reported for the protamine 1 and 2. MaterialsAndMethods The aim of this study was to evaluate the frequency of two previously repo...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2013
ISSN: 1470-8175
DOI: 10.1002/bmb.20705